The purpose of this clinical trial was to compare the effectiveness and safety of a new dental flossing device (Quik Floss) to finger flossing in plaque removal, reduction of gingival inflammation and patient preference. 30 healthy, non-flossing adults with 20 natural teeth, a plaque index (PI) of at least 1.8 and a gingival index (GI) of 1.3 or greater participated in this two-phase crossover design study. Patients were randomly assigned to use either the new flossing device or finger flossing first in the sequence. At baseline, data were collected and patients were given flossing method instructions. The Silness & Löe GI, Quigley-Hein PI, Eastman Bleeding Index (BI) and trauma were assessed at baseline, 15 and 30 days for each treatment period. After the initial 30-day treatment phase, patients were instructed to cease all flossing during a 14-day "washout" period. Subjects were then assigned to the opposite treatment group during the 2nd treatment phase. A 3-factor repeated measures ANOVA analyzed data for differences within and between groups over time. Results revealed statistically significant (p < 0.01) within group improvement in PI, GI, and BI for both treatment groups over time. No statistically significant between group differences were found for either group at any time period. In addition, no significant gingival trauma was recorded and no differences were found between groups. Quik Floss was as effective as finger flossing. Subjects preferred (55%) Quik Floss over finger flossing. In conclusion, Quik Floss is a safe and effective oral hygiene measure for reducing plaque, inflammation and bleeding over time.