Structure and expression of Leydig insulin-like peptide mRNA in the sheep

Mol Cell Endocrinol. 1996 Aug 9;121(2):171-7. doi: 10.1016/0303-7207(96)03861-0.


We have used PCR to isolate and characterise Leydig insulin-like peptide (Ley I-L) mRNA from sheep ovary. The deduced amino acid sequence of sheep Ley I-L has good homology with the pig and human peptides, having 93% and 77% amino acid identity, respectively. Northern blot analysis revealed abundant expression in both ovary and testis. An examination of ovarian RNA from non-pregnant and pregnant sheep showed that pregnancy did not significantly increase Ley I-L mRNA levels. However mRNA levels did alter depending on whether ovaries contained a corpus luteum. Also ovaries were examined by hybridisation histochemistry to locate the site of expression. Abundant Ley I-L mRNA levels were found in the theca interna cells of the ovary.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Female
  • Gene Expression Regulation
  • Humans
  • Insulin
  • Molecular Sequence Data
  • Organ Specificity
  • Ovary / metabolism
  • Polymerase Chain Reaction
  • Pregnancy
  • Proteins / genetics*
  • Proteins / metabolism
  • RNA, Messenger / genetics*
  • Sheep
  • Swine


  • Insulin
  • Leydig insulin-like protein
  • Proteins
  • RNA, Messenger