Apoptosis of mouse naive T cells induced by recombinant soluble Fas ligand and activation-induced resistance to Fas ligand

J Immunol. 1996 Nov 1;157(9):3918-24.


We have investigated the susceptibility of mouse T cells at various maturation and activation stages to recombinant soluble mouse Fas ligand (termed WX1). CD4+CD8+ thymocytes were preferentially killed by WX1, although CD4+CD8- and CD4-CD8+ thymocytes were also killed at higher concentrations of WX1. The majority of CD4-CD8- thymocytes were resistant to the same doses of WX1. Naive peripheral T cells were also killed by WX1 at concentrations similar to those for single-positive thymocytes. Activation-induced cell death of previously activated T cells is known to be mediated by Fas and Fas ligand. However, the activation of naive splenic T cells by mAbs against CD3/TCR complex rather induced resistance against WX1. This resistance was induced in activated T cells but not in bystander T cells. These mechanisms seem to explain why activated T cells are not immediately killed and why bystander T cells are not activated in an Ag-nonspecific manner.

Publication types

  • Case Reports
  • Comparative Study

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • COS Cells
  • Cells, Cultured
  • DNA, Complementary / genetics
  • Drug Resistance
  • Fas Ligand Protein
  • Female
  • Humans
  • Lymphocyte Activation*
  • Membrane Glycoproteins / deficiency
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / pharmacology*
  • Mice
  • Mice, Inbred C3H
  • Mice, Mutant Strains
  • Receptor-CD3 Complex, Antigen, T-Cell / immunology
  • Recombinant Fusion Proteins / pharmacology
  • Signal Transduction
  • Species Specificity
  • T-Lymphocyte Subsets / drug effects*
  • T-Lymphocyte Subsets / immunology
  • Transfection


  • DNA, Complementary
  • FASLG protein, human
  • Fas Ligand Protein
  • Fasl protein, mouse
  • Membrane Glycoproteins
  • Receptor-CD3 Complex, Antigen, T-Cell
  • Recombinant Fusion Proteins