Cloning of minimally divergent allelic forms of the natural killer (NK) receptor Ly-49C, differentially controlled by host genes in the MHC and NK gene complexes

J Immunol. 1996 Nov 1;157(9):3936-42.


We have investigated whether expression of Ly-49C, detected by the mAb SW5E6, is subject to similar regulation by host MHC as is Ly-49A. Ly-49C expression was regulated by host MHC differentially in mice of A/Sn (A) and C57BL/6 (B) origin. Analysis of AXB recombinant inbred strains suggested two loci regulating the expression, one segregating with the NK gene complex and the other with the MHC. In MHC congenic strains, the A form of Ly-49C had a low expression level and was further down-regulated in the presence of the H-2b haplotype (very low). The B form of Ly-49C had a high expression level and was down-regulated in the presence of H-2Kk (medium). To test the hypothesis that the Ly-49C receptor in A/Sn and C57BL/6 mice may be differentially regulated by MHC products because they represent allelic forms, cDNAs for Ly-49C were cloned by reverse-transcriptase PCR from A/Sn and C57BL/6 mouse strains. The Ly-49C sequences from the two strains differed by six nucleotides leading to four predicted amino acid changes. Both cDNAs could be detected by the Ly-49C-specific Ab when expressed in EL-4 cells. Thus, like Ly-49A, Ly-49C expression is regulated by MHC class I molecules. Furthermore, A/Sn and C57BL/6 mice express different forms of Ly-49C that are down-regulated by different MHC class I molecules.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Amino Acid Sequence
  • Animals
  • Antigens, Ly*
  • Cloning, Molecular
  • Crosses, Genetic
  • DNA, Complementary / genetics
  • Gene Expression Regulation*
  • Genes*
  • H-2 Antigens / genetics*
  • Haplotypes / genetics
  • Killer Cells, Natural / metabolism*
  • Lectins, C-Type
  • Major Histocompatibility Complex / genetics*
  • Membrane Glycoproteins / biosynthesis
  • Membrane Glycoproteins / genetics*
  • Mice
  • Mice, Inbred Strains / genetics*
  • Molecular Sequence Data
  • Receptors, NK Cell Lectin-Like
  • Recombinant Fusion Proteins / biosynthesis
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transfection


  • Antigens, Ly
  • DNA, Complementary
  • H-2 Antigens
  • Lectins, C-Type
  • Membrane Glycoproteins
  • Receptors, NK Cell Lectin-Like
  • Recombinant Fusion Proteins

Associated data

  • GENBANK/U56404
  • GENBANK/U56405