Molecular cloning of Elk-3, a new member of the Ets family expressed during mouse embryogenesis and analysis of its transcriptional repression activity

DNA Cell Biol. 1996 Oct;15(10):855-62. doi: 10.1089/dna.1996.15.855.


We isolated a cDNA clone, Elk-3, that encodes a novel Ets transcription factor from 16-day mouse embryos. The deduced amino acid sequence of the protein was homologous to human ELK-1 and SAP-1. This protein, ELK-1, and SAP-1 shared some unique structural properties such as an Ets DNA-binding site in the amino-terminal region, a serum response factor interacting domain and phosphorylation sites of serine or threonine residues in the carboxy-terminal region. Northern blotting weakly revealed that two transcripts of 4 and 2.1 kb are expressed in the adult ovary and lung and a 2.1-kb transcript predominated in 8- to 14-day embryos. We assayed the transcriptional activities of Elk-3 protein on the cytokeratin EndoA enhancer containing Ets binding sites in endodermal cells. Elk-3 protein strongly repressed enhancer activity but did not affect the activity of the basal promoter in the absence of the enhancer. Furthermore, Elk-3 can suppress the activity of Ets-2 as the transcriptional activator on the EndoA enhancer. These data suggested that the Elk-3 gene product plays a role in transcriptional regulation during embryogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • DNA / metabolism
  • DNA, Complementary
  • DNA-Binding Proteins / chemistry
  • Embryo, Mammalian
  • Embryonic and Fetal Development*
  • Female
  • Gene Expression Regulation, Developmental*
  • Humans
  • Lung / metabolism
  • Mice
  • Molecular Sequence Data
  • Multigene Family
  • Oncogene Proteins*
  • Ovary / metabolism
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins / chemistry
  • Proto-Oncogene Proteins c-ets
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Sequence Homology, Amino Acid
  • Transcription Factors / biosynthesis*
  • Transcription Factors / chemistry
  • Transcription, Genetic*
  • ets-Domain Protein Elk-1
  • ets-Domain Protein Elk-4


  • DNA, Complementary
  • DNA-Binding Proteins
  • ELK1 protein, human
  • ELK4 protein, human
  • Elk1 protein, mouse
  • Elk3 protein, mouse
  • Elk4 protein, mouse
  • Oncogene Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-ets
  • Recombinant Proteins
  • Transcription Factors
  • ets-Domain Protein Elk-1
  • ets-Domain Protein Elk-4
  • DNA

Associated data

  • GENBANK/S82864