Murine leukemia virus-based Tat-inducible long terminal repeat replacement vectors: a new system for anti-human immunodeficiency virus gene therapy

J Virol. 1996 Nov;70(11):8234-40. doi: 10.1128/JVI.70.11.8234-8240.1996.

Abstract

We have constructed new murine leukemia virus (MLV)-based vectors (TIN vectors) which, following integration, contain human immunodeficiency virus (HIV) type 1 U3 and R sequences in place of the MLV U3 and R regions. This provides, for the first time, single transcriptional unit retroviral vectors under the control of Tat. TIN vectors have several advantages for anti-HIV gene therapy applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Base Sequence
  • Cell Line
  • DNA, Viral
  • Gene Products, tat / genetics
  • Gene Products, tat / metabolism*
  • Genetic Therapy / methods*
  • Genetic Vectors*
  • HIV Infections / therapy*
  • HIV Long Terminal Repeat*
  • HIV-1 / genetics*
  • HeLa Cells
  • Humans
  • Leukemia Virus, Murine / genetics*
  • Mice
  • Molecular Sequence Data
  • tat Gene Products, Human Immunodeficiency Virus

Substances

  • DNA, Viral
  • Gene Products, tat
  • tat Gene Products, Human Immunodeficiency Virus