Abstract
We have constructed new murine leukemia virus (MLV)-based vectors (TIN vectors) which, following integration, contain human immunodeficiency virus (HIV) type 1 U3 and R sequences in place of the MLV U3 and R regions. This provides, for the first time, single transcriptional unit retroviral vectors under the control of Tat. TIN vectors have several advantages for anti-HIV gene therapy applications.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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Base Sequence
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Cell Line
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DNA, Viral
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Gene Products, tat / genetics
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Gene Products, tat / metabolism*
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Genetic Therapy / methods*
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Genetic Vectors*
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HIV Infections / therapy*
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HIV Long Terminal Repeat*
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HIV-1 / genetics*
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HeLa Cells
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Humans
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Leukemia Virus, Murine / genetics*
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Mice
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Molecular Sequence Data
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tat Gene Products, Human Immunodeficiency Virus
Substances
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DNA, Viral
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Gene Products, tat
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tat Gene Products, Human Immunodeficiency Virus