A meiotic recombination checkpoint controlled by mitotic checkpoint genes

Nature. 1996 Oct 31;383(6603):840-3. doi: 10.1038/383840a0.

Abstract

In budding yeast, meiotic recombination occurs at about 200 sites per cell and involves DNA double-strand break (DSB) intermediates. Here we provide evidence that a checkpoint control requiring the mitotic DNA-damage checkpoint genes RAD17, RAD24 and MEC1 ensures that meiotic recombination is complete before the first meiotic division (MI). First, RAD17, RAD24 and MEC1 are required for the meiotic arrest caused by blocking the repair of DSBs with a mutation in the recA homologue DMC1. Second, mec1 and rad24 single mutants (DMC1+) appear to undergo MI before all recombination events are complete. Curiously, the mitosis-specific checkpoint gene RAD9 is not required for meiotic arrest of dmc1 mutants. This shows that although mitotic and meiotic control mechanisms are related, they differ significantly. Rad17 and Rad24 proteins may contribute directly to formation of an arrest signal by association with single-strand DNA in mitosis and meiosis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Cycle Proteins*
  • DNA
  • DNA Damage
  • DNA Repair
  • DNA, Fungal / genetics
  • DNA-Binding Proteins
  • Fungal Proteins / genetics
  • Intracellular Signaling Peptides and Proteins
  • Meiosis / genetics*
  • Mitosis / genetics*
  • Mutagenesis
  • Nuclear Proteins
  • Protein-Serine-Threonine Kinases
  • Recombination, Genetic*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*

Substances

  • Cell Cycle Proteins
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • RAD17 protein, S cerevisiae
  • RAD24 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • DNA
  • MEC1 protein, S cerevisiae
  • Protein-Serine-Threonine Kinases