Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci

Plant J. 1996 Oct;10(4):655-61. doi: 10.1046/j.1365-313x.1996.10040655.x.


Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA-deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Abscisic Acid / biosynthesis
  • Abscisic Acid / genetics*
  • Arabidopsis / drug effects
  • Arabidopsis / genetics*
  • Arabidopsis / radiation effects
  • Chromosome Mapping
  • Crosses, Genetic
  • Darkness
  • Dose-Response Relationship, Drug
  • Drug Resistance
  • Genes, Plant*
  • Genes, Recessive
  • Germination
  • Light
  • Meiosis
  • Mutation*
  • Polyethylene Glycols / pharmacology
  • Selection, Genetic
  • Sodium Chloride / pharmacology
  • Triazoles / pharmacology
  • Water / metabolism


  • Triazoles
  • Water
  • Polyethylene Glycols
  • Sodium Chloride
  • paclobutrazol
  • Abscisic Acid