Characterization and mapping of Ds-GUS-T-DNA lines for targeted insertional mutagenesis

Plant J. 1996 Oct;10(4):721-32. doi: 10.1046/j.1365-313x.1996.10040721.x.


The transposition patterns of the Ds-GUS transposon T-DNA in 23 independent single-copy lines have been characterized and the map positions of 10 of them on three of the five Arabidopsis chromosomes are reported. Using overexpressed Activator (Ac) elements as a transposase source, it was found that the primary determinant of transposition frequency is the insertion site of the Ac-T-DNA. Neither the structure of the transposon T-DNA nor, in most cases, its insertion site have a significant effect on transposition frequency. Both the frequency and timing of transposition are influenced by the parent through which the transposon and transposase T-DNAs are transmitted. Overall, nearly 75% of plants in which excision has occurred bear a reinserted element and very short-range transpositions predominate, underlining the advantage of using mapped transposons for insertional mutagenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arabidopsis / genetics*
  • Chromosome Mapping
  • Crosses, Genetic
  • DNA Nucleotidyltransferases / genetics
  • DNA Transposable Elements / genetics*
  • Gene Dosage
  • Genes, Reporter
  • Genetic Markers
  • Germ Cells
  • Glucuronidase / genetics
  • Mutagenesis, Insertional / methods*
  • Plant Roots / growth & development
  • Recombination, Genetic*
  • Transposases


  • DNA Transposable Elements
  • Genetic Markers
  • DNA Nucleotidyltransferases
  • Transposases
  • Glucuronidase