Central Infusions of Leptin and GLP-1-(7-36) Amide Differentially Stimulate c-FLI in the Rat Brain

Am J Physiol. 1996 Oct;271(4 Pt 2):R1096-100. doi: 10.1152/ajpregu.1996.271.4.R1096.

Abstract

Recently, glucagon-like peptide-1-(7-36) amide (GLP-1) and leptin have been implicated in the regulation of food intake. In the present study, we compared the effects of third ventricular administration (i3vt) of leptin (3.5 micrograms) and GLP-1 (10.0 micrograms) on short-term food intake and c-Fos-like immunoreactivity (c-FLI) in hypothalamic, limbic, and hindbrain areas in the rat. Relative to controls, infusion of leptin or GLP-1 (3 h before lights off) significantly reduced food intake over the first 2 h in the dark phase (53 and 63%, respectively). In different rats, infusion of leptin or GLP-1 elevated c-FLI in the paraventricular hypothalamus and central amygdala. Furthermore, leptin selectively elevated c-FLI in the dorsomedial hypothalamus, whereas GLP-1 selectively elevated c-FLI in the nucleus of the solitary tract, area postrema, lateral parabrachial nucleus, and arcuate hypothalamic nucleus. The fact that most of the c-FLI after leptin or GLP-1 administration was observed in separate regions within the central nervous system (CNS) suggests different roles for leptin and GLP-1 in the CNS regulation of food intake and body weight.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain / metabolism
  • Brain / physiology*
  • Eating / drug effects
  • Glucagon
  • Glucagon-Like Peptide 1
  • Glucagon-Like Peptides
  • Immunohistochemistry
  • Injections, Intraventricular
  • Leptin
  • Male
  • Peptide Fragments / pharmacology*
  • Proteins / pharmacology*
  • Proto-Oncogene Proteins c-fos / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Tissue Distribution

Substances

  • Leptin
  • Peptide Fragments
  • Proteins
  • Proto-Oncogene Proteins c-fos
  • glucagon-like peptide 1 (7-36)amide
  • Glucagon-Like Peptides
  • Glucagon-Like Peptide 1
  • Glucagon