Expression of the gene for tear lipocalin/von Ebner's gland protein in human prostate

FEBS Lett. 1996 Oct 21;395(2-3):95-8. doi: 10.1016/0014-5793(96)01008-3.


Northern analysis of human multiple tissue blots containing poly A+ RNA from spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes revealed that a prostate specific transcript hybridizes to a tear lipocalin/von Ebner's gland protein (TL/VEGP) gene probe. To characterize this transcript, the corresponding cDNA was amplified by reverse transcription (RT)-PCR. Cloning and sequence analysis showed that it was identical to the tear lipocalin cDNA isolated from human lachrymal glands. Immunohistochemical analysis on thin layer sections of human prostate using a tear lipocalin specific antiserum confirmed the expression of this cDNA in prostate. Thus, our results clearly argue against a unique function of TL/VEGP in human tear fluid or saliva. The human cDNA was expressed in E. coli using the pQE system yielding a recombinant protein which shows biochemical properties identical to the native TL/VEGP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / genetics
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary
  • Escherichia coli
  • Female
  • Humans
  • Intestinal Mucosa / metabolism
  • Lipocalin 1
  • Male
  • Molecular Sequence Data
  • Organ Specificity
  • Ovary / metabolism
  • Polymerase Chain Reaction
  • Prostate / metabolism*
  • RNA, Messenger / biosynthesis
  • Recombinant Proteins / biosynthesis
  • Salivary Proteins and Peptides / biosynthesis*
  • Salivary Proteins and Peptides / genetics
  • Spleen / metabolism
  • Testis / metabolism
  • Thymus Gland / metabolism
  • Transcription, Genetic*


  • Carrier Proteins
  • DNA Primers
  • DNA, Complementary
  • LCN1 protein, human
  • Lipocalin 1
  • RNA, Messenger
  • Recombinant Proteins
  • Salivary Proteins and Peptides