Ectopic expression of CHOP (GADD153) induces apoptosis in M1 myeloblastic leukemia cells

FEBS Lett. 1996 Oct 21;395(2-3):143-7. doi: 10.1016/0014-5793(96)01016-2.


CHOP (GADD153) is a member of the C/EBP family and a stress-induced protein. To investigate the role of CHOP in cellular growth, we expressed CHOP conditionally in M1 myeloblastic leukemia cells that do not express p53 protein. More than 60% of M1 cells died through apoptosis 72 h after CHOP induction. Site-directed mutagenesis revealed that this process requires leucine zipper domain but neither intact basic region nor trans-activation domain. CHOP-mediated apoptosis accompanied downregulation of bcl-2 mRNA and overexpression of Bcl-2 delayed the process. Our results indicate that CHOP can induce apoptosis in a p53-independent manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis*
  • CCAAT-Enhancer-Binding Proteins*
  • Cell Line
  • Cloning, Molecular
  • Cricetinae
  • DNA Damage
  • DNA-Binding Proteins / biosynthesis*
  • Genes, bcl-2
  • Isopropyl Thiogalactoside / pharmacology
  • Kinetics
  • Leukemia, Myeloid, Acute
  • Mice
  • Mutagenesis, Site-Directed
  • Nuclear Proteins / biosynthesis
  • Proto-Oncogene Proteins c-bcl-2 / biosynthesis
  • RNA, Messenger / biosynthesis
  • Recombinant Proteins / biosynthesis
  • Transcription Factor CHOP
  • Transcription Factors / biosynthesis*
  • Transcription, Genetic / drug effects
  • Transfection
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / biosynthesis
  • Tumor Suppressor Protein p53 / metabolism


  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • Ddit3 protein, mouse
  • Nuclear Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Messenger
  • Recombinant Proteins
  • Transcription Factors
  • Tumor Suppressor Protein p53
  • Transcription Factor CHOP
  • Isopropyl Thiogalactoside