Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family

Cell. 1996 Nov 1;87(3):427-36. doi: 10.1016/s0092-8674(00)81363-x.


We identified and cloned a cellular mediator of herpes simplex virus (HSV) entry. Hamster and swine cells resistant to viral entry became susceptible upon expression of a human cDNA encoding this protein, designated HVEM (for herpesvirus entry mediator). HVEM was shown to mediate the entry of several wild-type HSV strains of both serotypes. Anti-HVEM antibodies and a soluble hybrid protein containing the HVEM ectodomain inhibited HVEM-dependent infection but not virus binding to cells. Mutations in the HSV envelope glycoprotein gD significantly reduced HVEM-mediated entry. The contribution of HVEM to HSV entry into human cells was demonstrable in activated T cells. HVEM, the first identified mediator of HSV entry, is a new member of the TNF/NGF receptor family.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CHO Cells / metabolism
  • CHO Cells / virology
  • Cell Line
  • Chlorocebus aethiops
  • Cloning, Molecular
  • Cricetinae
  • Cricetulus
  • DNA, Complementary / genetics
  • Genes*
  • HeLa Cells / metabolism
  • HeLa Cells / virology
  • Humans
  • Molecular Sequence Data
  • Receptors, Tumor Necrosis Factor*
  • Receptors, Tumor Necrosis Factor, Member 14
  • Receptors, Virus / genetics
  • Receptors, Virus / isolation & purification
  • Receptors, Virus / physiology*
  • Simplexvirus / physiology*
  • Swine
  • T-Lymphocytes / metabolism
  • T-Lymphocytes / virology
  • Transfection
  • Vero Cells / metabolism
  • Vero Cells / virology


  • DNA, Complementary
  • Receptors, Tumor Necrosis Factor
  • Receptors, Tumor Necrosis Factor, Member 14
  • Receptors, Virus
  • TNFRSF14 protein, human

Associated data

  • GENBANK/U70321