Dual Roles for Patched in Sequestering and Transducing Hedgehog

Cell. 1996 Nov 1;87(3):553-63. doi: 10.1016/s0092-8674(00)81374-4.

Abstract

Secreted proteins of the Hedgehog (Hh) family have diverse organizing roles in animal development. Recently, a serpentine protein Smoothened (Smo) has been proposed as a Hh receptor. Here, we present evidence that implicates another multiple-pass transmembrane protein, Patched (Ptc), in Hh reception and suggests a novel signal transduction mechanism in which Hh binds to Ptc, or a Ptc-Smo complex, and thereby induces Smo activity. Our results also show that Ptc limits the range of Hh action; we provide evidence that high levels of Ptc induced by Hh serve to sequester any free Hh and therefore create a barrier to its further movement.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Animals, Genetically Modified
  • Drosophila Proteins*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / growth & development
  • Gene Expression Regulation, Developmental
  • Hedgehog Proteins
  • Insect Hormones / genetics
  • Insect Hormones / physiology*
  • Insect Proteins / physiology
  • Macromolecular Substances
  • Membrane Proteins / genetics
  • Membrane Proteins / physiology*
  • Models, Biological
  • Multigene Family
  • Proteins / genetics
  • Proteins / metabolism*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / physiology*
  • Receptors, G-Protein-Coupled*
  • Signal Transduction / physiology*
  • Smoothened Receptor
  • Wings, Animal / abnormalities
  • Wings, Animal / embryology
  • Wings, Animal / growth & development

Substances

  • Drosophila Proteins
  • Hedgehog Proteins
  • Insect Hormones
  • Insect Proteins
  • Macromolecular Substances
  • Membrane Proteins
  • Proteins
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Smoothened Receptor
  • dpp protein, Drosophila
  • ptc protein, Drosophila
  • smo protein, Drosophila
  • hh protein, Drosophila