Cyclo-oxygenase-2 messenger RNA induction in focal cerebral ischemia

J Cereb Blood Flow Metab. 1996 Nov;16(6):1366-72. doi: 10.1097/00004647-199611000-00035.


We have characterized the induction of the mitogen-inducible form of cyclo-oxygenase, COX-2, during focal cerebral ischemia following permanent middle cerebral artery occlusion (MCAO) in the rat. Marked unilateral induction of COX-2 mRNA was detected in ischemic regions ipsilateral to the occlusion. A significant increase in COX-2 mRNA was detected in "core" and "penumbra" regions of the cerebral cortex between 4 and 24 h after occlusion; this was most marked at 4 h in the penumbra region, in which a 19-fold increase above untreated control levels was detected. A smaller but significant induction was also detected at 4 h in the caudate. A correlation was demonstrated between the extent of COX-2 mRNA induction in cortical regions at 4 h and the severity of tissue damage subsequently detected at 24 h post MCAO. MK-801 significantly attenuated the induction of COX-2 mRNA in the penumbra region at 4 h. The demonstration of COX-2 induction following experimental ischemia highlights the importance of this reaction and its products and by-products, for example, free radicals, in the tissue response to this insult.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Ischemia / enzymology*
  • Cyclooxygenase 2
  • Dizocilpine Maleate / pharmacology
  • Isoenzymes / biosynthesis*
  • Male
  • Neuroprotective Agents / pharmacology
  • Prostaglandin-Endoperoxide Synthases / biosynthesis*
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / drug effects
  • Rats
  • Rats, Sprague-Dawley


  • Isoenzymes
  • Neuroprotective Agents
  • RNA, Messenger
  • Dizocilpine Maleate
  • Cyclooxygenase 2
  • Prostaglandin-Endoperoxide Synthases