TNF-mediated cytotoxicity and resistance in human prostate cancer cell lines

Prostate. 1996 Nov;29(5):296-302. doi: 10.1002/(SICI)1097-0045(199611)29:5<296::AID-PROS4>3.0.CO;2-8.


Background: The contribution of TNF receptor (TNF-R) expression was investigated with respect to TNF sensitivity or insensitivity for androgen-dependent and androgen-independent human prostate cancer (PCA) cell lines, respectively.

Methods: Flow cytometric analyses using monoclonal antibodies against the 55-kDa receptor (TNF-R1) and the 75-kDa receptor (TNF-R2) indicated that both receptors were expressed on all three cell lines.

Results: Moreover, expression of TNF-R1 was greater than expression of TNF-R2 in these PCA cells. All three PCA cell lines produced IL-6. However, IL-6 production was enhanced when TNF-insensitive JCA-1 and PC-3 cells, but not TNF-sensitive LNCaP cells, were treated with rTNF (10(-9) M).

Conclusions: These data suggest that the lack of an antiproliferative effect of rTNF on the androgen-independent PCA cell lines PC-3 and JCA-1 is not due to the failure of these cells to express TNF-R, but may be related to the differences in TNF-mediated IL-6 expression by these PCA cell lines.

MeSH terms

  • Androgens / pharmacology
  • Antibodies, Monoclonal / pharmacology
  • Binding Sites
  • Cell Division
  • Drug Resistance, Neoplasm*
  • Flow Cytometry
  • Gene Expression
  • Humans
  • Interferon-gamma / pharmacology
  • Interleukin-6 / biosynthesis
  • Male
  • Prostatic Neoplasms / drug therapy*
  • Prostatic Neoplasms / pathology
  • RNA, Messenger / metabolism
  • Receptors, Tumor Necrosis Factor / genetics
  • Receptors, Tumor Necrosis Factor / physiology*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / pharmacology
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism
  • Tumor Necrosis Factor-alpha / pharmacology*


  • Androgens
  • Antibodies, Monoclonal
  • Interleukin-6
  • RNA, Messenger
  • Receptors, Tumor Necrosis Factor
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma