Neomycin resistance as a selectable marker in Methanococcus maripaludis

Appl Environ Microbiol. 1996 Nov;62(11):4233-7. doi: 10.1128/aem.62.11.4233-4237.1996.

Abstract

We cloned the aminoglycoside phosphotransferase genes APH3'I and APH3'II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Cloning, Molecular
  • Drug Resistance, Microbial / genetics*
  • Genes, Bacterial
  • Genetic Markers
  • Kanamycin Kinase
  • Methanococcus / drug effects*
  • Methanococcus / enzymology
  • Methanococcus / genetics*
  • Neomycin / pharmacology*
  • Phosphotransferases (Alcohol Group Acceptor) / genetics
  • Plasmids / genetics
  • Promoter Regions, Genetic
  • Terminator Regions, Genetic
  • Transformation, Genetic

Substances

  • Anti-Bacterial Agents
  • Genetic Markers
  • Phosphotransferases (Alcohol Group Acceptor)
  • Kanamycin Kinase
  • Neomycin