Abstract
We show that members of the POU homeodomain family are among the transcription factors expressed in developing mouse skeletal muscle. From a cDNA library prepared from fetal muscle mRNA, we cloned a cDNA identical to that of Brn-4, a POU class II gene previously cloned from neural tissues. In limb muscle, we found that Brn-4 mRNA expression was highest at embryonic days 15-18, declined-after birth, and was undetectable in adults. The mRNAs of two additional POU genes, Emb (POU class VI) and Oct-1 (POU class II), were also expressed in developing muscle and, unlike Brn-4, continued to be expressed in postnatal and adult muscles. In skeletal muscle, expression of Brn-4 is myogenin-dependent, because muscles from myogenin-deficient fetuses contained much less Brn-4 mRNA than muscles from myogenin-expressing littermates. In contrast, expression of Emb was the same in the presence or absence of myogenin. The distinct pattern of Brn-4 mRNA expression and its dependence on a myogenic regulatory factor suggest that Brn-4 is part of the network of interacting transcription factors that control muscle-specific gene expression during mammalian myogenesis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Animals
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Cells, Cultured
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DNA, Complementary / genetics
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology*
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Extremities / embryology
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Gene Expression Regulation, Developmental*
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Genes, Homeobox*
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Genotype
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Homeodomain Proteins / biosynthesis
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Homeodomain Proteins / classification
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Homeodomain Proteins / genetics
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Homeodomain Proteins / physiology*
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Host Cell Factor C1
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Mice
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Mice, Inbred C3H
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Mice, Knockout
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Mice, Mutant Strains
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Multigene Family*
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Muscle Denervation
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Muscle Proteins / biosynthesis
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Muscle Proteins / genetics
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Muscle Proteins / physiology*
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Muscle, Skeletal / embryology*
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Muscle, Skeletal / metabolism
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Muscle, Skeletal / physiology
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Myogenin / genetics
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Myogenin / physiology*
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Nerve Tissue Proteins / biosynthesis
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / physiology*
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Octamer Transcription Factor-1
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Organ Specificity
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POU Domain Factors
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RNA, Messenger / genetics
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Recombinant Fusion Proteins / biosynthesis
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Regeneration
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Transcription Factors / biosynthesis
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Transcription Factors / genetics
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Transcription Factors / physiology*
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Transfection
Substances
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DNA, Complementary
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DNA-Binding Proteins
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Hcfc1 protein, mouse
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Homeodomain Proteins
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Host Cell Factor C1
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Muscle Proteins
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Myog protein, mouse
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Myogenin
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Nerve Tissue Proteins
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Octamer Transcription Factor-1
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POU Domain Factors
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Pou2f1 protein, mouse
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Pou3f4 protein, rat
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Pou6f1 protein, mouse
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RNA, Messenger
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Recombinant Fusion Proteins
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Transcription Factors
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Pou3f4 protein, mouse