Cloning and nucleotide sequence of the mphB gene for macrolide 2'-phosphotransferase II in Escherichia coli

FEMS Microbiol Lett. 1996 Nov 1;144(2-3):197-202. doi: 10.1111/j.1574-6968.1996.tb08530.x.


Macrolide 2'-phosphotransferase II [MPH(2')II] inactivates macrolide antibiotics. The mphB gene for MPH(2')II was cloned from Escherichia coli and sequenced. Analysis of the nucleotide sequence indicated that mphB encoded a protein of 302 amino acids with a molecular mass of 34483 Da. The carboxy terminal region of the deduced protein contained a sequence that resembled a conserved functional domain in aminoglycoside phosphotransferases.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli Proteins
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Phosphotransferases (Alcohol Group Acceptor) / genetics*
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid


  • Bacterial Proteins
  • Escherichia coli Proteins
  • Recombinant Fusion Proteins
  • Phosphotransferases (Alcohol Group Acceptor)
  • macrolide 2'-kinase
  • mphB protein, E coli

Associated data

  • GENBANK/D85892