Chromosome 1 Aneusomy With 1p36 Under-Representation Is Related to Histologic Grade, DNA Aneuploidy, High C-Erb B-2 and Loss of bcl-2 Expression in Ductal Breast Carcinoma

Int J Cancer. 1996 Oct 21;69(5):381-5. doi: 10.1002/(SICI)1097-0215(19961021)69:5<381::AID-IJC5>3.0.CO;2-1.

Abstract

Chromosome 1 abnormalities with loss of 1p36 have been investigated in 95 breast-cancer samples by means of a dual-target fluorescence in-situ hybridization (FISH) technique using the pUC 1.77 and p1-79 probes, specific for the 1q12 and 1p36 regions, respectively. Abnormalities for one or both probes were detected in 83/95 samples. Relative 1p36 under-representation was found in 79/95. The clinical relevance of these alterations was studied by comparing the FISH results with several parameters currently used in breast-cancer pathology. Distinct patterns of chromosome 1 abnormalities were found among the histologic types of breast carcinoma. Lobular or mucinous samples showed few or no alterations, whereas most ductal samples had high chromosome 1 polysomy with under-representation of 1p36. In ductal carcinomas, chromosome 1 alterations increased with histologic grade, DNA aneuploidy, loss of bcl-2 and high c-erb B-2 expression. These associations were found to be statistically significant. No correlation between chromosome 1 alterations and nuclear grade, age, size, lymph-node involvement, hormonal receptor presence, proliferation activity or p53 protein expression was detected. These results indicate the utility of this FISH technique for a better definition of the biological characteristics of ductal carcinomas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aneuploidy
  • Breast Neoplasms / chemistry
  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology*
  • Carcinoma, Ductal, Breast / chemistry
  • Carcinoma, Ductal, Breast / genetics
  • Carcinoma, Ductal, Breast / metabolism
  • Carcinoma, Ductal, Breast / pathology*
  • Cell Division
  • Chromosome Deletion
  • Chromosomes, Human, Pair 1 / genetics*
  • Female
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lymphocytes / chemistry
  • Middle Aged
  • Proto-Oncogene Proteins c-bcl-2 / biosynthesis*
  • Receptor, ErbB-2 / biosynthesis*
  • Receptors, Estrogen / analysis
  • Receptors, Progesterone / analysis
  • Tumor Suppressor Protein p53 / biosynthesis

Substances

  • Proto-Oncogene Proteins c-bcl-2
  • Receptors, Estrogen
  • Receptors, Progesterone
  • Tumor Suppressor Protein p53
  • Receptor, ErbB-2