Increased matrix metalloproteinase-9 secretion in short-term tissue cultures of prostatic tumor cells

Int J Cancer. 1996 Oct 21;69(5):386-93. doi: 10.1002/(SICI)1097-0215(19961021)69:5<386::AID-IJC6>3.0.CO;2-1.


We have examined the expression of 2 tumor-associated metalloproteinases, MMP-2 and MMP-9, in 48 primary cultures of prostatic carcinoma (PRCA) and 33 cultures of benign prostatic hyperplasia (BPH). PRCA cultures secrete significantly more MMP-9 than their benign counterparts. Secreted MMP-2 did not differ significantly in cultures but was lower in PRCA cultures. Two cultures of benign origin exhibited high MMP-9 secretion and growth patterns consistent with a malignancy. Both cases were followed and successively re-evaluated histologically and rediagnosed as organ-confined PRCA. MMP expression in culture may be of predictive value in the identification of incidental PRCA. MMP-9 secretion and its ratio with MMP-2 were highest in epithelial cultures from invasive, metastatic tumors when compared both to disease confined to prostate gland and to locally extensive disease. MMP-9 secretion was greatest also in cultures derived from tissues of high Gleason histological grade. Active MMP-9 species were detected in 15 cultures (31%) of PRCA. Active MMP-2 species were observed in cultures of both BPH and PRCA origin in almost the same amounts. Although average levels were not significantly different, as a ratio to proform species, a significant elevation was observed in cultures of PRCA origin. We propose, therefore, that an elevated expression of MMP-9 and a high ratio of MMP-9 to MMP-2 in short-term prostate epithelial cultures is of potential diagnostic and prognostic significance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Diagnosis, Differential
  • Dose-Response Relationship, Drug
  • Epidermal Growth Factor / pharmacology
  • Epithelium / metabolism
  • Humans
  • Male
  • Metalloendopeptidases / analysis
  • Metalloendopeptidases / drug effects
  • Metalloendopeptidases / metabolism*
  • Neoplasm Staging
  • Prostatic Neoplasms / classification
  • Prostatic Neoplasms / diagnosis*
  • Prostatic Neoplasms / enzymology
  • Tumor Cells, Cultured


  • Epidermal Growth Factor
  • Metalloendopeptidases