Changes in superoxide dismutase activity in liver and lung of old rats

Free Radic Res. 1996 Nov;25(5):401-5. doi: 10.3109/10715769609149062.


Superoxide dismutase activity was measured in liver and lung from 3 and 24 month-old rats. Both total SOD and Mn-SOD activity decreased significantly in the liver of old rats. Recent results from our laboratory have indicated that during aging, the activity of Cu/Zn-SOD decreases in rat liver and that there is an accumulation of altered protein. It was also shown that the old Cu/Zn-SOD had one histidine fewer than the young one. In the present study, the immunoprecipitation experiments showed that the amount of immunoprecipitable Mn-SOD from liver of old rats was greater than from young ones, but when amino acid residues were measured in purified young and old Mn-SOD from liver, no change was observed. In lung, no significant age-related differences in total SOD, Cu/Zn-SOD and Mn-SOD activity were found, nor was there accumulation of altered protein during aging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging*
  • Amino Acids / analysis
  • Animals
  • Liver / enzymology*
  • Lung / enzymology*
  • Male
  • Manganese
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / analysis
  • Superoxide Dismutase / metabolism*


  • Amino Acids
  • Manganese
  • Superoxide Dismutase