Purification of human RNA polymerase II and general transcription factors

Methods Enzymol. 1996;274:72-100. doi: 10.1016/s0076-6879(96)74009-0.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Western
  • Cell Nucleus / metabolism
  • Chromatography, Affinity / methods
  • Chromatography, Ion Exchange / methods
  • Cloning, Molecular
  • DNA-Binding Proteins / isolation & purification*
  • DNA-Binding Proteins / metabolism
  • Electrophoresis, Polyacrylamide Gel / methods
  • Escherichia coli
  • HeLa Cells
  • Humans
  • Indicators and Reagents
  • Molecular Weight
  • Protein Denaturation
  • RNA Polymerase II / isolation & purification*
  • RNA Polymerase II / metabolism
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Sequence Tagged Sites
  • TATA Box
  • TATA-Box Binding Protein
  • Transcription Factor TFIIB
  • Transcription Factor TFIID
  • Transcription Factor TFIIH
  • Transcription Factors / isolation & purification*
  • Transcription Factors / metabolism
  • Transcription Factors, TFII / isolation & purification

Substances

  • DNA-Binding Proteins
  • Indicators and Reagents
  • Recombinant Proteins
  • TATA-Box Binding Protein
  • Transcription Factor TFIIB
  • Transcription Factor TFIID
  • Transcription Factors
  • Transcription Factors, TFII
  • transcription factor TFIIE
  • Transcription Factor TFIIH
  • RNA Polymerase II
  • transcription factor TFIIF