Fluorescence anisotropy: rapid, quantitative assay for protein-DNA and protein-protein interaction

Methods Enzymol. 1996;274:492-503. doi: 10.1016/s0076-6879(96)74039-9.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Composition
  • Base Sequence
  • Binding Sites
  • Chromatography, Affinity
  • DNA / chemistry*
  • DNA / metabolism*
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / metabolism*
  • DNA-Directed RNA Polymerases / chemistry
  • DNA-Directed RNA Polymerases / isolation & purification
  • DNA-Directed RNA Polymerases / metabolism*
  • Escherichia coli / enzymology
  • Fluorescence Polarization / methods
  • Fluorescent Dyes
  • Kinetics
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / metabolism
  • Protein Binding
  • Transcription Factors / chemistry*
  • Transcription Factors / metabolism*

Substances

  • DNA-Binding Proteins
  • Fluorescent Dyes
  • Oligodeoxyribonucleotides
  • Transcription Factors
  • DNA
  • DNA-Directed RNA Polymerases