Short-term effect of erythropoietin on T-cell mitogenic proliferation in chronic renal failure patients

Nephron. 1996;72(1):27-9. doi: 10.1159/000188802.

Abstract

Uremic patients undergoing hemodialysis (HD) are known to be highly susceptible to infections. Recent data indicate that in addition to its well-known stimulating effects on red cell production, erythropoietin (EPO) may also have immunomodulating properties. The aim of this study was to examine the effect of EPO on lectin-induced T-lymphocyte transformation in uremic patients, as part of its effect on the immune response. Sixteen HD patients and 20 age- and sex-matched healthy controls were compared before and after 6 and 20 weeks of EPO treatment. T lymphocytes were analyzed for their mitogenic activity following treatment with phytohemagglutinin (PHA), concanavalin A (CON A) and anti-CD3 by measuring 3H-thymidine incorporation. HD patients showed reduced mitogenic responses to all mitogens tested, compared to healthy controls. During the 6 weeks of EPO administration, a significant increase in T-lymphocyte activity could be demonstrated following exposure to all three mitogens (PHA, from 32 +/- 2 to 45 +/- 8; CON A, from 11 +/- 3 to 25 +/- 9; anti-CD3, from 11 +/- 3 to 22 +/- 5, means +/- SD). This increase was augmented after 5 months of EPO treatment. We conclude therefore that EPO improves in vitro T-cell mitogenic proliferation, even after short periods of treatment.

MeSH terms

  • Adjuvants, Immunologic / metabolism
  • Adjuvants, Immunologic / pharmacology
  • Cell Division / drug effects
  • Erythropoietin / pharmacology*
  • Female
  • Hemoglobins / analysis
  • Humans
  • Kidney Failure, Chronic / complications
  • Kidney Failure, Chronic / metabolism*
  • Male
  • Middle Aged
  • Mitogens / pharmacology
  • Renal Dialysis
  • T-Lymphocytes / drug effects*
  • T-Lymphocytes / metabolism
  • Thymidine / metabolism
  • Transformation, Genetic / genetics
  • Uremia

Substances

  • Adjuvants, Immunologic
  • Hemoglobins
  • Mitogens
  • Erythropoietin
  • Thymidine