Abstract
Overexpression of B7 and intracellular adhesion molecule-1 (ICAM-1) on syngeneic cells triggered MHC-unrestricted lysis by MHC-restricted CD8+ CTL without TCR/CD3 engagement. Both CD28/B7 and LFA-1/ICAM-1 interactions were required for MHC-nonrestricted cytotoxicity. Cytotoxicity was measured in 4-h and 16- to 18-h cytotoxicity assays. B7-ICAM-1 overexpression triggered perforin- and Fas ligand-mediated lysis, while TNF-alpha was not elicited in MHC-unrestricted cytotoxicity. These results suggest that target cells may elicit MHC-unrestricted lysis from CTL through up-regulation of membrane adherence and costimulatory receptors.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
B7-1 Antigen / pharmacology*
-
CD28 Antigens / pharmacology
-
Cytotoxicity, Immunologic / drug effects*
-
Disease Susceptibility
-
Fas Ligand Protein
-
Immunization / methods
-
Intercellular Adhesion Molecule-1 / pharmacology*
-
Ligands
-
Lymphocyte Function-Associated Antigen-1 / pharmacology
-
Major Histocompatibility Complex / genetics*
-
Membrane Glycoproteins / drug effects
-
Membrane Glycoproteins / genetics
-
Mice
-
Mice, Inbred C57BL
-
Mice, Mutant Strains
-
Perforin
-
Pore Forming Cytotoxic Proteins
-
T-Lymphocytes, Cytotoxic / drug effects*
-
Up-Regulation / drug effects*
Substances
-
B7-1 Antigen
-
CD28 Antigens
-
Fas Ligand Protein
-
Fasl protein, mouse
-
Ligands
-
Lymphocyte Function-Associated Antigen-1
-
Membrane Glycoproteins
-
Pore Forming Cytotoxic Proteins
-
Perforin
-
Intercellular Adhesion Molecule-1