Fucosyltransferase III and sialyl-Le(x) expression correlate in cultured colon carcinoma cells but not in colon carcinoma tissue

Glycoconj J. 1996 Oct;13(5):727-33. doi: 10.1007/BF00702336.


The potential contribution of fucosyltransferases to the overexpression of sialyl-Le(x) antigen was investigated in the colon carcinoma cell line HT-29 and in human colon carcinoma tissue. In HT-29 cells as well as in normal or malignant colonic tissues Fuc-TIII, Fuc-TIV, Fuc-TVI but not Fuc-TV nor Fuc-TVII were detectable after RT-PCR. Sodium butyrate treatment of HT-29 cells increased (to about 200%) and DMSO treatment decreased (to about 20%) the expression of sialyl-Le(x). This modulation of sialyl-Le(x) was concomitant with the analogous increase/decrease of mRNA of Fuc-TIII but not Fuc-TIV. Fuc-TVI was not detectable by Northern blotting in HT-29 cells. In six human colon carcinomas which exhibited strong overexpression of sialyl-Le(x), the expression of Fuc-TIII-mRNA was the same or lower than in the corresponding normal colonic tissue. Thus Fuc-TIII expression may be affecting the expression of the sialyl-Le(x) moiety in HT-29 cells but not in human colon carcinoma tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / metabolism
  • Blotting, Northern
  • Butyrates / pharmacology
  • Butyric Acid
  • Colonic Neoplasms / enzymology
  • Colonic Neoplasms / metabolism
  • Dimethyl Sulfoxide / pharmacology
  • Fucosyltransferases / metabolism*
  • Gene Expression Regulation, Neoplastic / genetics*
  • Humans
  • Lewis X Antigen / biosynthesis
  • Lewis X Antigen / metabolism*
  • Polymerase Chain Reaction
  • RNA, Messenger / metabolism
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • Butyrates
  • Lewis X Antigen
  • RNA, Messenger
  • Butyric Acid
  • Fucosyltransferases
  • Dimethyl Sulfoxide