Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 274 (5291), 1367-71

Structure of the A Site of Escherichia Coli 16S Ribosomal RNA Complexed With an Aminoglycoside Antibiotic

Affiliations

Structure of the A Site of Escherichia Coli 16S Ribosomal RNA Complexed With an Aminoglycoside Antibiotic

D Fourmy et al. Science.

Abstract

Aminoglycoside antibiotics that bind to 30S ribosomal A-site RNA cause misreading of the genetic code and inhibit translocation. The aminoglycoside antibiotic paromomycin binds specifically to an RNA oligonucleotide that contains the 30S subunit A site, and the solution structure of the RNA-paromomycin complex was determined by nuclear magnetic resonance spectroscopy. The antibiotic binds in the major groove of the model A-site RNA within a pocket created by an A-A base pair and a single bulged adenine. Specific interactions occur between aminoglycoside chemical groups important for antibiotic activity and conserved nucleotides in the RNA. The structure explains binding of diverse aminoglycosides to the ribosome, their specific activity against prokaryotic organisms, and various resistance mechanisms, and provides insight into ribosome function.

Similar articles

See all similar articles

Cited by 177 articles

See all "Cited by" articles

Publication types

MeSH terms

Associated data

LinkOut - more resources

Feedback