The novel protein-tyrosine phosphatase PTP20 is a positive regulator of PC12 cell neuronal differentiation

J Biol Chem. 1996 Nov 15;271(46):29422-6. doi: 10.1074/jbc.271.46.29422.


A novel cytoplasmic protein-tyrosine phosphatase (PTPase) designated PTP20 was isolated from a PC12 cDNA library and shown to positively regulate the differentiation process in PC12 cells. The PTP20 open reading frame of 453 amino acids contains a single tyrosine phosphatase catalytic domain and displays closest homology to members of the PTP-PEST protein-tyrosine phosphatase family. Transient expression of PTP20 in Rat-1 cells resulted in the expression of a 50-kDa protein which exhibited PTPase activity in vitro. Expression of the 2.3-kilobase PTP20 mRNA increased during differentiation of nerve growth factor (NGF)-stimulated PC12 cells. Consistent with this observation, stable overexpression of PTP20 in PC12 cells resulted in accelerated neurite formation following NGF treatment. These findings suggest a positive regulatory role of PTP20 in NGF-dependent neuronal differentiation of PC12 cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Retracted Publication

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Differentiation / drug effects
  • Cell Differentiation / genetics
  • Cloning, Molecular
  • DNA, Complementary
  • Epidermal Growth Factor / pharmacology
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Nerve Growth Factors / pharmacology
  • Neurons / cytology*
  • PC12 Cells
  • Protein Tyrosine Phosphatases / genetics
  • Protein Tyrosine Phosphatases / metabolism*
  • Rats


  • DNA, Complementary
  • Nerve Growth Factors
  • Epidermal Growth Factor
  • Protein Tyrosine Phosphatases

Associated data

  • GENBANK/U69673