A reliable and sensitive enzyme immunoassay method for measuring 8-isoprostaglandin F2 alpha: a marker for lipid peroxidation after experimental brain injury

J Neurosci Methods. 1996 Oct;68(2):133-6. doi: 10.1016/0165-0270(96)00014-3.


The objectives of this study were to determine (1) if levels of 8-isoprostaglandin F2 alpha (8-isoPGF2 alpha), a non-enzymatically produced prostaglandin, were increased after cortical contusion and (2) if enzyme immunoassay (EIA) could be used to quantify 8-isoPGF2 alpha levels. 24 h after the contusion there was a significant rise in 8-isoPGF2 alpha compared to control levels. The levels returned to baseline by 72 h postinjury. The results show that this EIA method is a reliable and sensitive technique for measuring brain lipid peroxidation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers
  • Brain Injuries / metabolism*
  • Dinoprostone / metabolism*
  • Immunoassay / methods*
  • Lipid Peroxidation / physiology*
  • Rats
  • Rats, Sprague-Dawley


  • Biomarkers
  • Dinoprostone