Evaluation of differentiation-inducing activity of retinoids on human leukemia cell lines HL-60 and NB4

Biol Pharm Bull. 1996 Oct;19(10):1322-8. doi: 10.1248/bpb.19.1322.


Retinoids, including all-trans-retinoic acid (ATRA), its isomers, and fifty synthetic retinoids (retinobenzoic acids), were tested for differentiation-inducing activity on human leukemia cell lines HL-60 and NB4. Binding activity of typical retinoids to nuclear retinoic acid receptors (RARs) was also investigated. A good linear correlation between the ED50 values of differentiation-inducing activity towards HL-60 cells and those towards NB4 cells was found. Binding activities of retinoids to RAR alpha and RAR beta also correlated well to the differentiation-inducing activities.

MeSH terms

  • Benzoates / pharmacology
  • Cell Differentiation / drug effects
  • HL-60 Cells
  • Humans
  • Receptors, Retinoic Acid / metabolism
  • Retinoic Acid Receptor alpha
  • Retinoids / pharmacology*
  • Structure-Activity Relationship
  • Trimethylsilyl Compounds / pharmacology


  • Benzoates
  • RARA protein, human
  • Receptors, Retinoic Acid
  • Retinoic Acid Receptor alpha
  • Retinoids
  • TAC 101
  • Trimethylsilyl Compounds
  • retinoic acid receptor beta