Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth

Genetics. 1996 Nov;144(3):967-78. doi: 10.1093/genetics/144.3.967.


Diploid strains of baker's yeast Saccharomyces cerevisiae can grow in a cellular yeast form or in filaments called pseudohyphae. This dimorphic transition from yeast to pseudohyphae is induced by starvation for nitrogen. Not all laboratory strains are capable of this dimorphic switch; many grow only in the yeast form and fail to form pseudohyphae when starved for nitrogen. Analysis of the standard laboratory strain S288C shows that this defect in dimorphism results from a nonsense mutation in the FLO8 gene. This defect in FLO8 blocks pseudohyphal growth in diploids, haploid invasive growth, and flocculation. Since feral strains of S. cerevisiae are dimorphic and have a functional FLO8 gene, we suggest that the flo8 mutation was selected during laboratory cultivation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Base Sequence
  • Cell Nucleus
  • DNA, Fungal
  • Fungal Proteins / genetics*
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development*
  • Saccharomyces cerevisiae Proteins*
  • Trans-Activators / genetics*


  • DNA, Fungal
  • FLO8 protein, S cerevisaie
  • Fungal Proteins
  • Nuclear Proteins
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators

Associated data

  • GENBANK/U51431