Ethylene receptor expression is regulated during fruit ripening, flower senescence and abscission

Plant Mol Biol. 1996 Sep;31(6):1227-31. doi: 10.1007/BF00040839.

Abstract

Using the Arabidopsis ethylene receptor ETR1 as a probe, we have isolated a tomato homologue (tETR) from a ripening cDNA library. The predicted amino acid sequence is 70% identical to ETR1 and homologous to a variety of bacterial two component response regulators over the histidine kinase domain. Sequencing of four separate cDNAs indicates that tETR lacks the carboxyl terminal response domain and is identical to that encoded by the tomato Never ripe gene. Ribonuclease protection showed tETR mRNA was undetectable in unripe fruit or pre-senescent flowers, increased in abundance during the early stages of ripening, flower senescence, and in abscission zones, and was greatly reduced in fruit of ripening mutants deficient in ethylene synthesis or response. These results suggest that changes in ethylene sensitivity are mediated by modulation of receptor levels during development.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Ethylenes / metabolism
  • Gene Expression Regulation, Plant*
  • Lycopersicon esculentum / genetics
  • Lycopersicon esculentum / growth & development*
  • Molecular Sequence Data
  • Plant Proteins / biosynthesis*
  • Plant Proteins / genetics
  • Plant Shoots / growth & development*
  • RNA, Messenger / biosynthesis
  • RNA, Plant / biosynthesis
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Cell Surface / genetics
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • Ethylenes
  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant
  • Receptors, Cell Surface
  • ethylene receptors, plant
  • ethylene

Associated data

  • GENBANK/Z54099