Enhanced CD34 expression of sinusoid-like vascular endothelial cells in hepatocellular carcinoma

Pathol Int. 1996 Oct;46(10):751-6. doi: 10.1111/j.1440-1827.1996.tb03544.x.


The immunohistochemical expression of CD34 (human hematopoietic stem cell and endothelial cell marker) and laminin were studied in chronic liver diseases and hepatocellular carcinoma (HCC) to elucidate whether their expression reflected phenotypic differences between non-cancerous sinusoids and sinusoid-like tumor vessels. In normal liver, hepatic sinusoids were always negative for CD34 and laminin. In chronic hepatitis and cirrhosis, the two antigens were sparsely expressed in capillarized sinusoids at periportal and perinodular area. In advanced HCC, CD34 was strongly and diffusely expressed by the endothelial lining of sinusoid-like tumor vessels. However, early-stage HCC showed a wide spectrum of CD34 expression from negative to focal and diffuse, strongly positive staining in sinusoid-like vessels. Laminin was strongly expressed in advanced HCC but not in early-stage HCC. The results indicate that the enhanced expression of CD34 by sinusoidal endothelial cells may reflect the phenotypic change of endothelial cells in chronic liver diseases and HCC, and that the expression may correlate with the processes of angiogenesis induced by hepatocarcinogenesis.

MeSH terms

  • Antigens, CD34 / biosynthesis*
  • Carcinoma, Hepatocellular / blood supply*
  • Carcinoma, Hepatocellular / metabolism*
  • Carcinoma, Hepatocellular / pathology
  • Endothelium, Vascular / immunology*
  • Endothelium, Vascular / metabolism*
  • Endothelium, Vascular / pathology
  • Hepatitis / immunology
  • Hepatitis / metabolism
  • Humans
  • Immunohistochemistry
  • Laminin / biosynthesis
  • Liver Circulation / immunology*
  • Liver Cirrhosis / immunology
  • Liver Cirrhosis / metabolism
  • Liver Neoplasms / blood supply*
  • Liver Neoplasms / metabolism*
  • Liver Neoplasms / pathology
  • Up-Regulation / immunology*


  • Antigens, CD34
  • Laminin