The early gene region completes the nucleotide sequence of Lactobacillus delbrueckii subsp. lactis phage LL-H

Gene. 1996 Oct 10;175(1-2):49-57. doi: 10.1016/0378-1119(96)00119-9.


Transcription of genes from phage LL-H can be divided into an early phase and a late phase. The early gene region was located in a 5.9-kb segment of the phage LL-H genome and it was part of the sequence that completed the phage LL-H genome sequence, 34 659 bp in size. Phage LL-H is the first completely sequenced Lactobacillus phage. In the main coding strand of phage LL-H genome 48 putative ORFs could be detected, but only four small putative ORFs could be found in the opposite strand. The ORFs covered 85.6% of the main coding strand. Function could be assigned to eleven of the phage LL-H ORFs either by biochemical analyses or by database homologies. A single-strand-binding protein, SSB, was detected in addition to the previously determined functions (small and large subunits of terminase, intron-encoded endonuclease, six structural proteins, phage lysin). For 15 additional ORFs of phage LL-H homology was detected in databases, but no function could be inferred for them.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / genetics*
  • Bacteriophages / pathogenicity
  • Base Sequence
  • Genes, Immediate-Early / genetics*
  • Genes, Viral / genetics*
  • Genome, Viral*
  • Lactobacillus / virology*
  • Molecular Sequence Data
  • Open Reading Frames / genetics*
  • Virulence / genetics

Associated data

  • GENBANK/L02496
  • GENBANK/L29567
  • GENBANK/L29568
  • GENBANK/L37351
  • GENBANK/L42315
  • GENBANK/L46882
  • GENBANK/L46883
  • GENBANK/M96254