Isolation of a Candida glabrata centromere and its use in construction of plasmid vectors

Gene. 1996 Oct 10;175(1-2):105-8. doi: 10.1016/0378-1119(96)00132-1.

Abstract

A centromere has been isolated from Candida glabrata by functional selection based on the lethality of the SUP11 gene at high copy number. Nucleotide sequence analysis revealed a centromeric structure similar to that of Saccharomyces cerevisiae: the two highly conserved elements CDEI (8 bp) and CDEIII (26 bp) are separated by a 79-bp A+T-rich element, CDEII. Three centromere-bearing plasmid vectors with different selection markers have been constructed. These plasmids were highly stable in mitosis (< 1% loss rate per generation) and exist in one or two copies per cell.

MeSH terms

  • Base Sequence
  • Candida / genetics*
  • Candida / pathogenicity
  • Centromere / genetics*
  • Conserved Sequence
  • DNA, Fungal / genetics
  • Genetic Vectors / genetics*
  • Molecular Sequence Data
  • Sequence Analysis, DNA

Substances

  • DNA, Fungal

Associated data

  • GENBANK/U43925
  • GENBANK/U43926