Characterization of the RNA dependent DNA polymerase of bovine leukemia virus

Anticancer Res. Sep-Oct 1996;16(5A):2501-5.

Abstract

The reverse transcriptase-RNA dependent DNA polymerase of Bovine Leukemia Virus (BLV) was isolated and characterized. The enzyme has a molecular weight of about 80kd and the isoelectric point is 7.6. The enzyme prefers magnesium, as a divalent cation using synthetic homopolymeric template primer poly (C) oligo (dG). Monoclonal antibodies directed against reverse transcriptase of human immunodeficiency virus type I (HIV-I) did not crossreact with the isolated polymerase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Cell Line
  • Cross Reactions
  • HIV Reverse Transcriptase / immunology
  • Leukemia Virus, Bovine / enzymology*
  • Molecular Weight
  • RNA-Directed DNA Polymerase / chemistry
  • RNA-Directed DNA Polymerase / immunology
  • RNA-Directed DNA Polymerase / isolation & purification*
  • Transcription, Genetic

Substances

  • Antibodies, Monoclonal
  • HIV Reverse Transcriptase
  • RNA-Directed DNA Polymerase