Abstract
We cloned and characterized a cDNA corresponding to a cdc5+ homolog of the higher plant, Arabidopsis thaliana. The cDNA, named AtCDC5 cDNA, encodes a polypeptide of 844 amino acid residues. The amino acid sequence of N-terminal one-fourth region of the predicted protein bears significant similarity to that of Schizosaccharomyces pombe Cdc5 and Myb-related proteins. Overexpression of the AtCDC5 cDNA in S. pombe cells is able to complement the growth defective phenotype of a cdc5 temperature-sensitive mutant. These results indicate that the AtCDC5 gene is a plant counterpart of S. pombe cdc5+. This is the first report of a cdc5(+)-like gene in a multicellular organism. We also demonstrated that a recombinant AtCDC5 protein possesses a sequence specific DNA binding activity (CTCAGCG) and the AtCDC5 gene is expressed extensively in shoot and root meristems. In addition, we cloned a PCR fragment corresponding to the DNA binding domain of human Cdc5-like protein. These results strongly suggest that Cdc5-like protein exists in all eukaryotes and may function in cell cycle regulation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins*
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Base Sequence
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Binding Sites
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Cell Cycle Proteins / biosynthesis*
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Cell Cycle Proteins / chemistry*
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Cell Cycle Proteins / metabolism
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DNA Primers
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Drosophila
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Genetic Complementation Test
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Glutathione Transferase / biosynthesis
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Humans
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In Situ Hybridization
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Plant Proteins / biosynthesis*
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Plant Proteins / chemistry
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Polymerase Chain Reaction
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Proto-Oncogene Proteins / chemistry
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Proto-Oncogene Proteins c-myb
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Recombinant Fusion Proteins / biosynthesis
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Schizosaccharomyces / genetics
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Schizosaccharomyces / growth & development
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Schizosaccharomyces pombe Proteins
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Sequence Homology, Amino Acid
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Trans-Activators / chemistry
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Transcription Factors / biosynthesis*
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Transcription Factors / chemistry
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Transcription Factors / metabolism
Substances
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ATCDC5 protein, Arabidopsis
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Arabidopsis Proteins
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Cell Cycle Proteins
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DNA Primers
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Oligodeoxyribonucleotides
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Plant Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-myb
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Recombinant Fusion Proteins
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Schizosaccharomyces pombe Proteins
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Trans-Activators
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Transcription Factors
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cdc5 protein, S pombe
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Glutathione Transferase
Associated data
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GENBANK/D58424
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GENBANK/D85423