Styrene metabolism in Exophiala jeanselmei and involvement of a cytochrome P-450-dependent styrene monooxygenase

Appl Environ Microbiol. 1996 Apr;62(4):1471-4. doi: 10.1128/aem.62.4.1471-1474.1996.


The yeast-like fungus Exophiala jeanselmei degrades styrene via initial oxidation of the vinyl side chain to phenylacetic acid, which is subsequently hydroxylated to homogentisic acid. The initial reactions are catalyzed by a NADPH- and flavin adenine dinucleotide-dependent styrene monooxygenase, a styrene oxide isomerase, and a NAD(+)-dependent phenylacetaldehyde dehydrogenase. The reduced CO-difference spectrum of microsomal preparations of styrene-grown cells shows a characteristic absorption maximum at 450 nm, which strongly suggests the involvement of a cytochrome P-450-dependent styrene monooxygenase. Inhibition of styrene monooxygenase activity in cell extracts by cytochrome P-450 inhibitors SKF-525-A, metyrapone, and CO confirms this assumption.

MeSH terms

  • Air Pollutants / metabolism
  • Biodegradation, Environmental
  • Carbon Monoxide / pharmacology
  • Cytochrome P-450 Enzyme Inhibitors
  • Cytochrome P-450 Enzyme System / metabolism
  • Enzyme Inhibitors / pharmacology
  • Exophiala / metabolism*
  • Metyrapone / pharmacology
  • Oxygenases / antagonists & inhibitors
  • Oxygenases / metabolism*
  • Proadifen / pharmacology
  • Spectrophotometry
  • Styrene
  • Styrenes / chemistry
  • Styrenes / metabolism*


  • Air Pollutants
  • Cytochrome P-450 Enzyme Inhibitors
  • Enzyme Inhibitors
  • Styrenes
  • Styrene
  • Carbon Monoxide
  • Cytochrome P-450 Enzyme System
  • Proadifen
  • Oxygenases
  • styrene monooxygenase
  • Metyrapone