Abstract
By comparing lists of 3'-directed partial cDNA sequences (gene signatures) randomly collected from various tissues, genes uniquely expressed in individual tissue can be identified. A full length cDNA clone, corresponding to one such gene signature, unique to human osteoblast and adipose tissue, was isolated. This cDNA clone encodes a 845-amino acid protein that is almost identical to the mouse adipocyte transcription factor AEBP1 except that it has additional 105 amino acids in the N terminus. A northern hybridization showed that in the differentiating murine osteoblastic cell line, AEBP1 is also expressed but that it is shut off in the final calcification phase, suggesting a transcriptional repressive effect on genes for bone formation.
MeSH terms
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3T3 Cells
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Adipocytes / metabolism
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Adipose Tissue / metabolism
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Amino Acid Sequence
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Animals
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Base Sequence
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Calcium-Binding Proteins
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Carboxypeptidases
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Cell Differentiation*
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Cell Line
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Cells, Cultured
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Cloning, Molecular
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DNA, Complementary
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Female
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Humans
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Intercellular Signaling Peptides and Proteins
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Membrane Proteins / biosynthesis*
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Membrane Proteins / chemistry*
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Mice
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Molecular Sequence Data
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Organ Specificity
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Osteoblasts / cytology
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Osteoblasts / metabolism*
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Pregnancy
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Repressor Proteins / biosynthesis*
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Repressor Proteins / chemistry*
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Sequence Homology, Amino Acid
Substances
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AEBP1 protein, human
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Calcium-Binding Proteins
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DLK1 protein, human
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DNA, Complementary
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Dlk1 protein, mouse
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Intercellular Signaling Peptides and Proteins
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Membrane Proteins
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Repressor Proteins
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Carboxypeptidases
Associated data
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GENBANK/D86479
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PIR/S51739