Associations of UBE2I With RAD52, UBL1, p53, and RAD51 Proteins in a Yeast Two-Hybrid System

Genomics. 1996 Oct 15;37(2):183-6. doi: 10.1006/geno.1996.0540.

Abstract

The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the "bait" in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 16
  • DNA Repair
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Humans
  • Ligases / genetics
  • Ligases / metabolism*
  • Male
  • Molecular Sequence Data
  • Protein Binding
  • RNA, Messenger / metabolism
  • Rad51 Recombinase
  • Rad52 DNA Repair and Recombination Protein
  • SUMO-1 Protein
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins*
  • Testis / metabolism
  • Tumor Suppressor Protein p53 / metabolism*
  • Ubiquitin-Conjugating Enzymes*

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • Fungal Proteins
  • RAD52 protein, S cerevisiae
  • RAD52 protein, human
  • RHP51 protein, S pombe
  • RNA, Messenger
  • Rad52 DNA Repair and Recombination Protein
  • SUMO-1 Protein
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Tumor Suppressor Protein p53
  • Ubiquitin-Conjugating Enzymes
  • RAD51 protein, S cerevisiae
  • RAD51 protein, human
  • Rad51 Recombinase
  • Ligases
  • HUS5 protein, S pombe
  • ubiquitin-conjugating enzyme UBC9

Associated data

  • GENBANK/M74525
  • GENBANK/U38785