Cloning of Human Lymphocyte-Specific Interferon Regulatory Factor (hLSIRF/hIRF4) and Mapping of the Gene to 6p23-p25

Genomics. 1996 Oct 15;37(2):229-33. doi: 10.1006/geno.1996.0547.

Abstract

The interferon regulatory factor (IRF) genes encode a family of transcription factors involved in the transcriptional regulation of interferon and the interferon stimulated genes through recognition of the interferon stimulated response element. We previously reported the cloning of a murine lymphocyte-specific IRF (mLSIRF), which was rapidly induced following B- or T-cell receptor crosslinking. To study the role of LSIRF in human lymphocyte development, we have cloned the complete 5.3-kb cDNA for the human homolog (hLSIRF). hLSIRF is a protein of 450 amino acids with a predicted molecular weight of 51.6 kDa and possesses 92% identity at the amino acid level to mLSIRF, including near identity in the DNA-binding domain. In Northern blot analysis, a single transcript of approximately 5 kb was highly expressed in spleen and peripheral blood lymphocyte. hLSIRF mRNA was rapidly induced in peripheral T cells after crosslinking the T-cell receptor. Analysis of tumor cell lines showed that hLSIRF mRNA was basally expressed in most B- but not T-cell lines. Surprisingly hLSIRF mRNA was also found in the melanoma line G361 and is expressed in normal melanocytes as well. Sequence from a genomic clone for hLSIRF was compared to that from mouse and revealed an identical exon-intron structure and a conserved PU-1-binding motif in the promoter. By FISH analysis, hLSIRF was mapped to 6p23-p25.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 6*
  • Cloning, Molecular
  • DNA, Complementary
  • DNA-Binding Proteins / genetics*
  • HeLa Cells
  • Humans
  • In Situ Hybridization, Fluorescence
  • Interferon Regulatory Factors
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Transcription Factors / genetics*
  • Tumor Cells, Cultured

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • Interferon Regulatory Factors
  • Transcription Factors
  • interferon regulatory factor-4

Associated data

  • GENBANK/N25007
  • GENBANK/N35268
  • GENBANK/U20949
  • GENBANK/U52682
  • GENBANK/U52683