A common-lines based method for determining orientations for N > 3 particle projections simultaneously

P A Penczek; J Zhu; J Frank

doi:10.1016/0304-3991(96)00037-x

A common-lines based method for determining orientations for N > 3 particle projections simultaneously

Ultramicroscopy. 1996 Jul;63(3-4):205-18. doi: 10.1016/0304-3991(96)00037-x.

Authors

P A Penczek¹, J Zhu, J Frank

Affiliation

¹ Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509, USA.

PMID: 8921628
DOI: 10.1016/0304-3991(96)00037-x

Abstract

A method is proposed for determining the directions of projections. An arbitrary number of projections of unknown three-dimensional structure are simultaneously used as input. The method is based on common lines and uses a new discrepancy measure accounting for the uneven distribution of common lines in angular space. An application to the 70S Escherichia coli ribosome data obtained from an energy-filtering electron microscope is described.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Algorithms
Escherichia coli / ultrastructure*
Image Processing, Computer-Assisted / methods*
Mathematics
Microscopy, Electron / methods*
Ribosomes / ultrastructure*

Grants and funding

1R 01 GM29169/GM/NIGMS NIH HHS/United States