Isolation and Characterization of the Haemophilus Influenzae uvrA Gene

Gene. 1996 Oct 24;177(1-2):23-8. doi: 10.1016/0378-1119(96)00264-8.

Abstract

The uvrA gene Haemophilus influenzae (Hi) was cloned and sequenced. Analysis of the deduced amino acid sequence revealed 81% identity and 90% similarity with the Escherichia coli UvrA protein. Consistent with a role of Hi uvrA in DNA repair, a Hi uvrA mutant exhibited increased sensitivity of UV irradiation. Furthermore, Hi uvrA was able to complement a mutation in the E. coli uvrA locus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics*
  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Cloning, Molecular
  • DNA Repair
  • DNA, Bacterial
  • DNA-Binding Proteins / genetics*
  • Escherichia coli Proteins*
  • Genes, Bacterial*
  • Genetic Complementation Test
  • Haemophilus influenzae / enzymology
  • Haemophilus influenzae / genetics*
  • Haemophilus influenzae / radiation effects
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Ultraviolet Rays

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • UvrA protein, E coli
  • Adenosine Triphosphatases

Associated data

  • GENBANK/U33877