Construction by homologous recombination and phenotypic characterization of a DNA polymerase domain polA mutant of Mycobacterium smegmatis

Gene. 1996 Oct 31;178(1-2):125-30. doi: 10.1016/0378-1119(96)00350-2.


Gene replacement was achieved by homologous recombination in Mycobacterium smegmatis (Ms) using a cloned segment of the polA gene (encoding the DNA polymerase I) disrupted within the region encoding the C-terminal DNA polymerase domain by a kanamycin-resistance marker. The Ms polA755:aph mutant thus generated displayed a phenotype of hypersensitivity to DNA damage induced by UV irradiation and by hydrogen peroxide challenge.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • DNA Damage
  • DNA Polymerase I / genetics*
  • DNA Polymerase I / metabolism
  • Gene Targeting
  • Hydrogen Peroxide / pharmacology
  • Molecular Sequence Data
  • Mutation
  • Mycobacterium / drug effects
  • Mycobacterium / enzymology*
  • Mycobacterium / genetics
  • Mycobacterium / radiation effects
  • Phenotype
  • Recombination, Genetic
  • Restriction Mapping


  • Hydrogen Peroxide
  • DNA Polymerase I

Associated data

  • GENBANK/U26405