Evaluation of four commercial enzyme immunoassays for detection of immunoglobulin M antibodies to human parvovirus B19

Eur J Clin Microbiol Infect Dis. 1996 Sep;15(9):758-61. doi: 10.1007/BF01691968.

Abstract

Four commercial enzyme immunoassays (EIAs) for the detection of parvovirus B19-specific immunoglobulin M (IgM) antibodies [Biotrin Parvovirus B19 IgM (Biotrin International, Ireland); Parvoscan B19 IgM (Euro-Diagnostica, Sweden); Parvovirus IgM (Immunobiological Laboratories [IBL], Germany); and human parvovirus B19 IgM (Hillcrest Biologicals, USA)] were compared to indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR). Using IFA as the reference test, high sensitivities (> or = 97%) were observed with all four EIAs, though the specificities of the Biotrin and IBL EIAs (99% and 96% respectively) were significantly higher than those of the Hillcrest and Euro-Diagnostica EIAs (81% and 79% respectively).

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Anti-Idiotypic / analysis*
  • Australia
  • Child, Preschool
  • Erythema Infectiosum / diagnosis*
  • Erythema Infectiosum / immunology
  • Evaluation Studies as Topic
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Immunoenzyme Techniques*
  • Immunoglobulin M*
  • Male
  • Parvovirus B19, Human / immunology
  • Parvovirus B19, Human / isolation & purification*
  • Polymerase Chain Reaction
  • Sensitivity and Specificity

Substances

  • Antibodies, Anti-Idiotypic
  • Immunoglobulin M