Genetic aspects of carbon catabolite repression of the STA2 glucoamylase gene in Saccharomyces cerevisiae

Yeast. 1996 Oct;12(13):1297-300. doi: 10.1002/(SICI)1097-0061(199610)12:13%3C1297::AID-YEA13%3E3.0.CO;2-U.


Three regulatory genes, known to be required for glucose repression/derepression of some genes in Saccharomyces cerevisiae, were disrupted to study their effects on the carbon-source regulation of the STA2 glucoamylase gene expression. Using a STA2-lacZ fusion it was found that: (1) the MIG1 gene is dispensable for the repression of the STA2 gene; (2) there are two components in the carbon-source repression of STA2: HXK2-dependent and HXK2-independent; and (3) the HAP2 gene seems to be involved in repression rather than activation of the STA2 expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • CCAAT-Binding Factor*
  • DNA-Binding Proteins / genetics
  • Fungal Proteins / genetics
  • Gene Expression Regulation, Enzymologic*
  • Gene Expression Regulation, Fungal*
  • Glucan 1,4-alpha-Glucosidase / genetics*
  • Glucose / metabolism
  • Lac Operon
  • Repressor Proteins / genetics*
  • Repressor Proteins / physiology*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors / genetics


  • CCAAT-Binding Factor
  • DNA-Binding Proteins
  • Fungal Proteins
  • MIG1 protein, S cerevisiae
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Glucan 1,4-alpha-Glucosidase
  • Glucose