Genomic organization and chromosomal mapping of the bovine Fas/APO-1 gene

DNA Cell Biol. 1996 May;15(5):377-85. doi: 10.1089/dna.1996.15.377.


The cell-surface protein Fas (APO-1) is a member of the tumor necrosis factor receptor (TNFR) superfamily and transduces apoptosis following binding of Fas ligand or exposure to certain anti-Fas antibodies. We have isolated the bovine Fas (bFas) gene and determined its genomic organization and chromosomal location. Our data indicate that bFas is a single-copy gene that contains 9 exons and spans approximately 31.5 kb. The 5'-flanking region lacks conventional TATA and CCAAT elements, but contains several putative regulatory elements, including multiple copies of Sp1, AP-2, E-box, and N-box consensus sites. Linkage analysis of two (CA) dinucleotide repeat microsatellites within intron 1 and physical assignment by fluorescence in situ hybridization (FISH) placed the bFas gene on bovine chromosome 26. Collectively, these data provide a basis for understanding the regulatory mechanisms that control bFas gene expression.

MeSH terms

  • Animals
  • Base Sequence
  • Cattle
  • Chromosome Mapping*
  • Cloning, Molecular
  • DNA / analysis
  • Dinucleotide Repeats / genetics
  • Exons / genetics
  • Gene Dosage
  • Genes / genetics*
  • Genetic Linkage
  • In Situ Hybridization, Fluorescence
  • Introns / genetics
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Transcription, Genetic / genetics
  • fas Receptor / genetics*


  • fas Receptor
  • DNA

Associated data

  • GENBANK/U24240
  • GENBANK/U24439
  • GENBANK/U24440