Sensitive determination of bromazepam in human tissues using capillary gas chromatography-mass spectrometry

J Chromatogr B Biomed Appl. 1996 Feb 23;677(1):111-6. doi: 10.1016/0378-4347(95)00427-0.

Abstract

A reliable and sensitive gas chromatographic-mass spectrometric method was devised to determine the levels of bromazepam in human tissues. Bromazepam was extracted from body tissues using a three-step solvent extraction procedure. N-Desmethyldiazepam served as the internal standard. Selected ion monitoring with m/z 317 for bromazepam and m/z 270 for internal standard was used for quantitation. Calibration curves in all body tissues were linear over the concentration range from 50-500 ng/g. The lower detection limit in body tissues was 2-5 ng/g and the absolute recovery in body tissues was 27.8-68.0%. This method was used to determine the levels of bromazepam in tissues of an autopsied individual who had been prescribed psychotropic drugs and who was found dead in a car.

MeSH terms

  • Anti-Anxiety Agents / analysis*
  • Anti-Anxiety Agents / pharmacokinetics
  • Brain Chemistry
  • Bromazepam / analysis*
  • Bromazepam / pharmacokinetics
  • Calibration
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Indicators and Reagents
  • Liver / chemistry
  • Reference Standards
  • Tissue Distribution

Substances

  • Anti-Anxiety Agents
  • Indicators and Reagents
  • Bromazepam