Differential expression of three glutamate transporter subtypes in the rat retina

Cell Tissue Res. 1996 Dec;286(3):325-36. doi: 10.1007/s004410050702.


The immunocytochemical distribution of the three excitatory amino acid transporter subtypes GLT-1, GLAST-1 and EAAC-1 was studied in the rat retina using antibodies raised against synthetic peptides corresponding to the C-terminus of each transporter subtype (Rothstein et al. 1994). A comparative immunoblot analysis of rat cortex, cerebellum and retina membrane proteins suggested the following rank order of glutamate transporter subtype expression in retina: GLAST-1>/=EAAC-1>GLT-1. GLAST-1 immunoreactivity was seen in Müller cells and astrocytes. EAAC-1 was found in horizontal cells, in amacrine and displaced amacrine cells, and in ganglion cells. A minority of bipolar cells also expressed EAAC-1. GLT-1 was preferentially expressed by different types of bipolar cells; however, it was also found in some amacrine cells. The functional role of this differential distribution of glutamate transporters in the retina is discussed.

MeSH terms

  • ATP-Binding Cassette Transporters / metabolism*
  • Amino Acid Transport System X-AG
  • Animals
  • Astrocytes / metabolism
  • Biological Transport
  • Blotting, Western
  • Calbindin 2
  • Calbindins
  • Carrier Proteins / metabolism*
  • Choline O-Acetyltransferase / metabolism
  • Eye Proteins / metabolism
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Glycoproteins / metabolism*
  • Male
  • Rats
  • Retina / cytology
  • Retina / metabolism*
  • S100 Calcium Binding Protein G / metabolism


  • ATP-Binding Cassette Transporters
  • Amino Acid Transport System X-AG
  • Calbindin 2
  • Calbindins
  • Carrier Proteins
  • Eye Proteins
  • Glycoproteins
  • S100 Calcium Binding Protein G
  • Choline O-Acetyltransferase